PCR Cloning Kits
ABclonal Technology PCR Enhancer for Microbiome
For certain difficult samples that cannot be effectively amplified or generate non-specific amplification products, using RK20725 PCR Enhancer for Microbiome with RK20720 TruePol 2X PCR Mix for Microbiome improve the specificity and success rate of amplification. RK20720 Truepol 2X PCR Mix for Microbiome is suitable for amplification of general and complex sample source templates, such as human tissue samples, soil samples, fecal samples, air filter membrane samples, sand samples, etc.
Cayman Chemical ANTIBODY WQ 1 5mg
A σ1 receptor antagonist (Ki = 0.01 nM); selective for σ1 receptor over σ2 receptor and DAT (Kis = 295.12 and 1,585 nM, respectively); decreases proliferation of MCF-7 and MCF-7/adr cancer cells at 10 µM; induces cell cycle arrest at the G0/G1 phase in MCF-7 and MCF-7/adr cancer cells and apoptosis in MCF-7/adr cancer cells at 10 µM; increases the duration of morphine-induced latency to tail withdrawal in the tail-flick test in mice at 1 mg/kg 30 minutes prior to the test; reduces stress-induced food consumption in rats at 3 or 7 mg/kg
INTACT GENOMICS INC ig-Fusion™ Cloning Enzyme Premix 100 Reactions
Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step. Cloning takes usually 10-30 min. with more than 95% cloning efficiency.Applications Clone any insert at any site within any vector Restriction enzyme and phosphatase free system Joining multiple large fragments at once Precise insertion at a desired orientation Rapid and high efficiency with > 95% positive clones
New England Biolabs, Inc. NEB PCR Cloning Kit – 20 reactions
This PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5 which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq, LongAmp Taq) which produce single base overhangs. Possible due to invisible end polishing components in the master mix that are active during the ligation step only if needed. Kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5'-phosphate groups. The ultimate in flexibility: clone with any amplicon made with any DNA polymerase, with or without 5' phosphates, purified or not! Includes Competent Cells.
VectorBuilder E. COLI STOCK CLONING
Vector cloning service for pLV[Exp]-EGFP:T2A:Puro#2;EF1A>hTRPC6[NM_004621.6] (VB900123-7495ykc
Apexbio Technology LLC Hyper Assembly Cloning Kit, 50 rxns. Cas: MFCD: NA. used for fast, directional cloning of one or more fragments of DNA into any vector.
Hyper Assembly Cloning Kit is designed for fast, directional cloning of one or more target fragment of DNA into any vector. This method employs the 3-5 exonuclease activity of Hyper Assembly Enzyme to get 15-20nt complementary arms at the ends of PCR-generated inserts and linearized vectors, then fuse the DNA fragments to get the recombinant vectors. These 15-20-bp overlaps can be engineered by designing primers for amplification of the desired sequences. Clone any insert into any location within any vector you choose Efficiently clone a broad range of fragment sizes Clone multiple DNA fragments simultaneously into any vector in a single reaction No restriction digestion, phosphatase treatment, or ligation required Other sizes are also available. Please inqury us for quote.
Revvity Health Sciences Inc Sealed Glass Chamber Assembly with 20 mm Probe, 473 mL
Sealed Glass Chamber Assembly with 20 mm Probe, 473 mL
Cayman Chemical ANTIBODY WQ 1 10mg
A σ1 receptor antagonist (Ki = 0.01 nM); selective for σ1 receptor over σ2 receptor and DAT (Kis = 295.12 and 1,585 nM, respectively); decreases proliferation of MCF-7 and MCF-7/adr cancer cells at 10 µM; induces cell cycle arrest at the G0/G1 phase in MCF-7 and MCF-7/adr cancer cells and apoptosis in MCF-7/adr cancer cells at 10 µM; increases the duration of morphine-induced latency to tail withdrawal in the tail-flick test in mice at 1 mg/kg 30 minutes prior to the test; reduces stress-induced food consumption in rats at 3 or 7 mg/kg
Mitegen Morpheus Fusion HT-96 Protein Crystallization Screen, 96 Conditions, Incomplete Factorial, For Stable & Membrane Proteins
The Morpheus Fusion HT-96 is a 96-condition, incomplete factorial protein crystallization screen specifically designed to uncover novel crystallization space using a range of low-molecular weight ligands. Its mild chemistry, based on PEG-precipitant mixes with no extreme pH, makes it highly suitable for samples with challenging stability, including macromolecular complexes and membrane proteins, as well as for soaking crystals with drug compounds. This comprehensive screen features 19 unique additive mixes, promoting initial crystal formation and enhancing lattice stability. All conditions are cryoprotected, reducing crystal stress and simplifying subsequent handling. The Morpheus Fusion HT-96 is engineered for easy optimization of 'hits', streamlining the path to high-quality crystals. Its advanced formulation accelerates the identification of optimal growth conditions, proving indispensable for researchers aiming to achieve reliable and reproducible structural data.